| iTEM Solution Tomography - Explore the full information depth of your specimens
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| Key features |
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- Automated tilt image series acquisition
- Automated and manual alignment
- Automated reconstruction
- 3-D reconstruction
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Summary
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Tomography using Transmission Electron Microscopes, or Electron Tomography, is developing into a more widely known and versatile method for obtaining TEM sample information from the third dimension. This 3-D information is of critical importance for understanding biological systems such as proteins, protein-nucleic acid complexes and macromolecular machines.
The iTEM Solution Tomography enables the user to obtain the desired results with the highest accuracy and reliability – with minimal user intervention. The user-friendly workflow including the automatic acquisition, alignment, reconstruction and visualization makes it very easy to achieve the best results from a tomographic sample – even for inexperienced users.
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| Product |
iTEM Solution Tomography
iTEM Solution Tomography
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Acquisition |
Calibration Wizard (6 steps) for easy adaption of goniometer/holder vs. software |
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Auto-correction of focus changes, illumination conditions and specimen movement |
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Supported tilting schemes: Linear and Saxton |
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Preadjustment of tilt axis for higher precision |
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Cryo- and Low-dose acquisition - under development |
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Alignment |
Automatic offline alignment |
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Automatic location of tilt axis (no fiducial markers needed) |
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Reconstruction |
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Automatic application of image filters |
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Supported reconstruction methods:
Weighted back-projection (WBP)
Simultaneous iterative reconstruction tomography (SIRT) – under development |
| Visualization |
SliceViewer: display of orthogonal section planes |
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VoxelViewer:
Intensity projections
Isosurface projection (with triangulation)
Blending projection with user-definable LUTs |
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Variable clipping plane for easy insight views |
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Measurements within 3-D model (positions, distances, polygons) |
| Supported Image Files |
Import: TIFF (also multi page), MRC, etc |
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Export: TIFF, AVI (eg, from 3-D model), Stereo RGB, etc |
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iTEM platform
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iTEM is the premier image analysis platform for TEM. It includes automation, processing, analysis, visualization, archiving, and report generation.
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Sales/Support
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If you have any questions, please feel free to contact us. Click the image or the link to find out about phone, fax, and email.
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